Phagocytosis assays with different pH-sensitive fluorescent particles and various readouts
نویسندگان
چکیده
منابع مشابه
High-throughput phagocytosis assay utilizing a pH-sensitive fluorescent dye.
We describe a development of a novel high-throughput phagocytosis assay based on a pH-sensitive cyanine dye, CypHer5E, which is maximally fluorescent in an acidic environment. This dye is ideally suited for the study of phagocytosis because of the acidic conditions generated in the intracellular phagocytic vesicles after particle uptake. Use of CypHer5E-labeled particles results in greatly redu...
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Many physiological processes function efficiently within a well-controlled pH range. Higher acidity level has been implicated with a number of systemic pathologies. The potential of pH sensitive fluorescent probes for reporting on biological environments has been widely utilized in a variety of cell studies and has been recently recognized as a powerful technique for in vivo imaging of diseases...
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Phagocytosis of pathogens is an important component of the innate immune system that is responsible for the removal and degradation of bacteria as well as their presentation via the major histocompatibility complexes to the adaptive immune system. The periodontal pathogen Porphyromonas gingivalis exhibits strain heterogeneity, which may affect a phagocyte's ability to recognize and phagocytose ...
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A quantum dot conjugated to a dye through an experimentally simple process of self-assembly exhibits an enhanced emission when the dye is attached, and this effect is pH-sensitive.
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We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reacti...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2020
ISSN: 0736-6205,1940-9818
DOI: 10.2144/btn-2020-0003